Molecular and Cellular Biologist

Select Source International

Cambridge, MA, USA

Published: 6/14/2022

Science

Full Time

Job Description

Job Title: Molecular and Cellular Biologist

Duration: 6 months

Location: Cambridge, MA 02139

We are seeking a scientist to join our Systems Immunology Lab in Client’s Inflammation & Immunology Research Unit. The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single cell transcriptomics technologies in collaboration with a small team.

Position Responsibilities:

· Generate custom lentiviral libraries and maintain extensive cell cultures. Generate edited pools of cells via lentiviral transduction and arrayed electroporation. Generate single-cell data via several commercial workflows, including from 10x Genomics. Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale.

· Reports to a lead scientist in Systems Immunology Lab. Collaborates with and supports several multi-disciplinary experimental teams on a per-project basis.

· Education and Experience: Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field

Description:

3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells, experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry.
Bonus: direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.

Hard Skills:

Diverse Cell Types
2. Molecular biology techniques *qPCR
3. Gene Editing

Preferred:

Direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing.

Interview Process: 2 Rounds, Video & In person

Company DescriptionSelect Source International (SSI) is a distinguished staffing company with nearly 25 years of experience, renowned for delivering top-tier workforce solutions encompassing staffing, recruiting, and managed services. As a proud supplier, we bring a wealth of expertise and best-in-business differentiators to comprehensively support your diverse workforce needs.

Based in Minneapolis, Minnesota, USA, SSI has established itself as a global leader, serving a broad clientele across industries with unwavering dedication and excellence. SSI is ISO 9001, 14001, and Soc2 Type2 certified, underscoring our commitment to quality and security. We have garnered numerous sustainability and supplier diversity awards, further validating our industry-leading capabilities

Company Description

Select Source International (SSI) is a distinguished staffing company with nearly 25 years of experience, renowned for delivering top-tier workforce solutions encompassing staffing, recruiting, and managed services. As a proud supplier, we bring a wealth of expertise and best-in-business differentiators to comprehensively support your diverse workforce needs.\r\n\r\nBased in Minneapolis, Minnesota, USA, SSI has established itself as a global leader, serving a broad clientele across industries with unwavering dedication and excellence. SSI is ISO 9001, 14001, and Soc2 Type2 certified, underscoring our commitment to quality and security. We have garnered numerous sustainability and supplier diversity awards, further validating our industry-leading capabilities